ectocervical epithelial cell line ect1 e6e7 (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC ectocervical epithelial cell line ect1 e6e7
Ectocervical Epithelial Cell Line Ect1 E6e7, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 349 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ectocervical+epithelial+cell+line+ect1+e6e7/Ect1%2FE6E7/pmc12157993-238-2-7
Average 96 stars, based on 349 article reviews

ectocervical epithelial cell line ect1 e6e7 - by Bioz Stars, 2026-09

96/100 stars

Images

Cell Culture:

Article Title: MiR-499a-5p Inhibits Proliferation, Invasion, Migration, and Epithelial–Mesenchymal Transition, and Enhances Radiosensitivity of Cervical Cancer Cells via Targeting eIF4E
Article Snippet: .. Human CC cell lines Hela, C-33A, SiHa, ME180, and CaSki and ectocervical epithelial cell line Ect1/E6E7 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 mg/mL streptomycin (all from Gibco, Carlsbad, CA, USA) in a humidified atmosphere with 5% CO 2 at 37°C. .. The miR-499a-5p mimic, miRNA negative control (miR-NC), siRNA against eIF4E (si-eIF4E), siRNA negative control (si-NC), pcDNA3.1-eIF4E plasmid (eIF4E) and the corresponding negative control (NC) were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China).

Article Title: Comparative studies on <i>in vitro</i> antitumor activities and apoptosis-inducing effects of enantiomeric ruthenium(II) complexes
Article Snippet: On the basis of our previous comparative studies on the DNA binding of a pair of ruthenium(II) complex enantiomers, Δ-[Ru(bpy)2PBIP] 2+ and Λ-[Ru(bpy)2PBIP] 2+ {bpy = 2,2’-bipyridine, PBIP = 2-(4-bromophenyl)imidazo[4,5-f ]1,10-phenanthroline}, in this study, their antitumor activities and mechanisms were further investigated comparatively.. The cytotoxicity assay demonstrated that both the enantiomers exerted selective antiproliferative effects on cancer cell lines A2780 and PC3.. Fluorescence localization experiments suggested that both the enantiomers effectively permeated the nucleus of HeLa cells and co-localized with DNA, resulting in their DNA damage and apoptosis.

Article Title: <p>MiR-499a-5p Inhibits Pr5/4/20 publish l Cancer Cells via Targeting eIF4E</p>
Article Snippet: .. Cell Culture Human CC cell lines Hela, C-33A, SiHa, ME180, and CaSki and ectocervical epithelial cell line Ect1/E6E7 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 mg/mL streptomycin (all from Gibco, Carlsbad, CA, USA) in a humidified atmosphere with 5% CO2 at 37°C. .. Cell Transfection and Treatment The miR-499a-5p mimic, miRNA negative control (miRNC), siRNA against eIF4E (si-eIF4E), siRNA negative control (si-NC), pcDNA3.1-eIF4E plasmid (eIF4E) and the corresponding negative control (NC) were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China).

Article Title: Trichomonas vaginalis extracellular vesicles activate the NLRP3 inflammasome and TLR3-mediated inflammatory cascades in host cells
Article Snippet: .. The human ectocervical epithelial cell line Ect1/E6E7 (ATCC CRL-2614) was cultured in keratinocyte serum-free medium (KSFM) (Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 0.1 ng/mL human recombinant epidermal growth factor, 0.05 mg/mL bovine pituitary extract, and 0.4 mM calcium chloride [ ]. .. The human monocyte cell line THP-1 (ATCC TIB-202) was cultured in Roswell Park Memorial Institute medium (RPMI 1640) supplemented with 10% fetal bovine serum (FBS) and incubated in 5% CO 2 at 37°C.

Article Title: Comparative studies on in vitro antitumor activities and apoptosis-inducing effects of enantiomeric ruthenium(II) complexes.
Article Snippet: Dalton Transactions An international journal of inorganic chemistry rsc.li/dalton ISSN 1477-9226 Volume 52 Number 40 28 October 2023 Pages 14245-14688 PAPER Hong Xu et al.. Comparative studies on in vitro antitumor activities and apoptosis-inducing eff ects of enantiomeric ruthenium( II ) complexes Dalton Transactions PAPER Cite this: Dalton Trans., 2023, 52, 14338 Received 25th May 2023, Accepted 29th June 2023 DOI: 10.1039/d3dt01584j rsc.li/dalton Comparative studies on in vitro antitumor activities and apoptosis-inducing effects of enantiomeric ruthenium(II) complexes Jianrong Jiang,†a,b,c,d,e,f Qian Chen,†a,b,c,d,e,f Tianwen Huan,a,b,c,d,e,f Yanhong Nie,a,b,c,d,e,f Zhongming Dai,g Dujuan Li, h Xu Xu, a,b,c,d,e,f Jun Lu,i Zhangli Hua,b,c,d,e,f,j and Hong Xu *a,b,c,d,e,f On the basis of our previous comparative studies on the DNA binding of a pair of ruthenium(II) complex enantiomers, Δ-[Ru(bpy)2PBIP] 2+ and Λ-[Ru(bpy)2PBIP] 2+ {bpy = 2,2’-bipyridine, PBIP = 2-(4-bromophenyl)imidazo[4,5-f ]1,10-phenanthroline}, in this study, their antitumor activities and mechanisms were further investigated comparatively.. The cytotoxicity assay demonstrated that both the enantiomers exerted selective antiproliferative effects on cancer cell lines A2780 and PC3.

Modification:

In Vitro:

Article Title: Fused deposition modeling three-dimensional printing of flexible polyurethane intravaginal rings with controlled tunable release profiles for multiple active drugs
Article Snippet: .. In vitro cytotoxicity studies were performed using the human vaginal epithelial cell line VK2/E6E7, ectocervical epithelial cell line Ect1/E6E7, and human T cell line SupT1 (ATCC, Rockville, MD, USA). .. The vaginal and ectocervical epithelial cells were cultured in keratinocyte-serum free medium (K-SFM) with 0.1 ng/mL of recombinant human epidermal growth factor, 50 mg/mL of bovine pituitary extract (Life Technologies, Carlsbad, CA, USA), 0.4 mM CaCl2 (VWR International, Batavia, IL, USA), and 1% penicillin/streptomycin (Sigma-Aldrich).

other:

Article Title: Impact of Hydroxychloroquine-Loaded Polyurethane Intravaginal Rings on Lactobacilli
Article Snippet: Medical-grade, nonbiodegradable, polyether urethane (PU) Tecophilic HP-60D-35 was purchased from Lubrizol (Wickliffe, OH, USA).

Recombinant:

Article Title: Immunomodulatory roles of autophagic flux and IFIT in human ectocervical cells upon Trichomonas vaginalis infection.
Article Snippet: The Tv strain (ATCC 30236) was maintained in YIS medium [30], pH 5.8, containing 10 % heat-inactivated horse serum and 1 % glucose at 37 ◦C. .. The human ectocervical epithelial cell line Ect1/E6E7 (ATCC CRL-2614TM) was maintained in keratinocyte-serum free medium (KSFM) (Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA) [31] supplemented with 0.1 ng/mL human recombinant epidermal growth factor, 0.05 mg/mL bovine pituitary extract, and 0.4 mM calcium chloride. ..

Review